Theresa May will make the case for her new Brexit plan in Parliament later, amid signs that Conservative opposition to her leadership is hardening.

The prime minister will give a statement to MPs on her changes to the Withdrawal Agreement Bill – including her promise to give MPs a vote on holding another referendum.

But Labour MPs said too little had changed for them to come on board.

And one ex-Tory minister questioned whether June’s vote would even happen.

MPs have rejected the withdrawal agreement negotiated with the EU three times, and attempts to find a formal compromise with Labour have failed.

On Tuesday, the prime minister asked MPs to take “one last chance” to deliver a negotiated exit – or risk Brexit not happening at all.

But Mrs May’s new plan had “failed to turn sceptics into endorsers”, said BBC political correspondent Chris Mason.

Conservative MP Boris Johnson – who wants to succeed Mrs May as prime minister – said on Twitter: “We are being asked to vote for a customs union and a second referendum. The Bill is directly against our manifesto – and I will not vote for it.

“We can and must do better – and deliver what the people voted for.”

Meanwhile Dominic Raab, another leadership hopeful, said Mrs May’s deal would “break our clear manifesto promises”.

Former Work and Pensions Secretary Stephen Crabb said the prospect of another referendum was too much for many of his colleagues.

“I don’t think there will be a vote,” he said. “It won’t pass. There is too much opposition.”

The 1922 backbench Conservative committee is to meet on Wednesday amid reports of fresh attempts to force another confidence vote in the PM’s leadership.

Tory MP Zac Goldsmith said it was time for Mrs May, who has already indicated she will leave office when the Brexit agreement is passed, to go now.

On Newsnight on Tuesday, backbench Tory MP Sir Bernard Jenkin said there was “a lot of affection and respect for Mrs May in the party – but a lot of anger at the mistakes that she has made”.

He said the process for a leadership election would be “accelerated”.

Meanwhile, DUP parliamentary leader Nigel Dodds said the main problem was the original deal Mrs May negotiated with the EU.

“We will examine the legislation closely when the bill is finally published but the fundamental flaws of the draft withdrawal agreement treaty itself remain unchanged,” he said.

“Many of the proposals on the backstop serve as an attempt through domestic law to mitigate a bad deal, whereas the focus should be on getting a better deal.”